The 5.56 mm NATO chambering, known as a NATO or mil-spec chamber, has a longer leade, which is the distance between the mouth of the cartridge and the point at which the rifling engages the bullet. The .223 Remington chambering, known as SAAMI chamber, is allowed to have a shorter leade, and is only required to be proof tested to the lower SAAMI chamber pressure. To address these issues, various proprietary chambers exist, such as the Wylde chamber (Rock River Arms) or the ArmaLite chamber, which are designed to handle both 5.56 mm NATO and .223 Remington equally well. The dimensions and leade of the .223 Remington minimum C.I.P. chamber also differ from the 5.56 mm NATO chamber specification.
Using commercial .223 Remington cartridges in a 5.56 mm NATO chambered rifle should work reliably, but generally will not be as accurate as when fired from a .223 Remington chambered gun due to the longer leade.